Synthetic Neurobiology Group

Ed Boyden, Ph.D., Principal Investigator

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Resources

Tools for mapping the molecular structure of the brain

  • Expansion microscopy: physical magnification with nanoscale precision

  • Pumby: programmable, modular RNA binding proteins

Tools for recording high-speed brain dynamics

  • SiRIs: spatial multiplexing of fluorescent reporters for imaging signaling network dynamics

  • SomaGCaMP: soma-targeted calcium indicators for precision neural population imaging

  • SomArchon: voltage indicator for population neural activity imaging

  • Archon1: well-localized, high-sensitivity, photostable fluorescent voltage indicator

  • Lightfield microscopy: fast 3-D neural imaging

  • Autopatching: robotic patch clamp in live brain

Optogenetics: molecules enabling neural control by light

  • ChromeQ: optogenetic activation with reduced proton and calcium currents

  • soCoChR: soma-targeted, high-amplitude channelrhodopsin

  • Jaws: red light-drivable halorhodopsin

  • Chrimson: red light-drivable channelrhodopsin

  • Chronos: high-speed, light-sensitive, channelrhodopsin

  • CoChR: large-current channelrhodopsin

  • Lumitoxins: light-actuated tethered toxin architecture

  • ArchT: light-sensitive archaerhodopsin

  • Arch: archaerhodopsin (light-driven proton pump)

  • Mac: light-driven proton pump of L. maculans

  • ChR2-2A-Halo: channelrhodopsin-halorhodopsin gene fusions

  • Halo/NpHR: halorhodopsin (light-driven chloride pump)

  • ChR2: channelrhodopsin-2 (light-driven cation channel)

  • Glial optogenetic tools

  • Accessory plasmids for optogenetics

  • Lentivirus production for high-titer, cell-specific, in vivo neural labeling

Optogenetics: hardware enabling neural control by light

  • Versatile, but Manually-Assembled, Optical Fiber/Laser System for In Vivo Optogenetics

  • Very Simple Off-The-Shelf Systems for In Vivo Optogenetics

Understanding normal and pathological brain computations

  • Closed-loop, ultraprecise, automated craniotomies

Home » Resources » Tools for recording high-speed brain dynamics » SomaGCaMP: soma-targeted calcium indicators for precision neural population imaging

SomaGCaMP: soma-targeted calcium indicators for precision neural population imaging

Publication [Shemesh OA*, Linghu C*, Piatkevich KD*, Goodwin D, Celiker OT, Gritton HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han X, Ahrens MB, Boyden ES (2019) Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator, Neuron 107(3):470-486. (*, equal contribution)]

Plasmids, plasmid maps, and plasmid preparation protocols, as well as viruses (AAV, lentivirus), available at [Addgene]

Viruses (AAV, lentivirus) available at [UNC vector core]

Viruses (AAV, lentivirus) available at [U. Penn vector core]

Contact [
Prof. Ed Boyden] with questions, comments, or to check for updates.

Projects

Tools for recording high-speed brain dynamics

Copyright 2006-present, Ed Boyden | Accessibility